Not known Lab Survey
Dr . Nathan Cahoone
Dec 9, 2010
There are many reasons for knowing the identity of microorganisms. The study and check was done by applying all the methods which were learned up to now in the microbiology laboratory school for the identification of your unknown bacterium which I was using not known #25. Benefits
Unknown #25 had this morphology on the streak platter: medium sized butyrous cream coloured colony. Gram-staining was used as a first step to identify between gram-positive and gram-negative. After deciding that it was a Gram adverse single bacillus, biochemical tests performed and the purpose and results are proven in desk and circulation chart kind. Table one particular: biochemical Test Results
Test| Purpose| Reagents| Observations| Outcomes
Gram stain| To determine the Gram result of the bacterium| Crystal purple, Iodine, Alcoholic beverages, Sarasin| Red rod| Gram-negative rods| Carbohydrate (Glucose) fermentation| To determine the capability of a bacteria to fremnet a carbohydrate, make chemical p or gas| Durham tube| Color change from red to yellow, bubble in Bowmanville tube| Great carbohydrate fermentation, acid creation and gas production| Tryptophan digestion| To look for the ability associated with an organism to split indole from tryptophan| Kovac's drop added to tryptone broth| Kind of colorless and list green ring in top of broth| Bad tryptophan digestion| Cystine digestion| To determine the ability of an affected person to catabolize certain amino acids| Flat iron ions| Color didn't transformed remaind creamy| Negative cystine digestion| Citrate Utilization| To determine the organism can utilize citrate as a carbon source| Citrate slant (green) & Bromthymol blue| Color changed via green to deep blue| Positive for citrate utilization| Urea digestion| To determine the ability of an organism to catabolize ammonia| Phenolmed| Color failed to change stayed at bright orange|...